Bio-Rad Immun-Blot® AP Colorimetric Kits User Manual
Page 13
Section 4
References
1.
Blake, M. S., Johnston, K. H., Russell-Jones, G. J. and Gotschlich, E. C.,
Anal. Biochem., 136, 175 (1984).
2.
Hawkes, R., Niday, E. and Gordon, J., Anal. Biochem., 119, 142 (1982).
3.
Towbin, H., Staehlin, T. and Gordon, J., PNAS, 76, 4350 (1979).
4.
Glass, W. F., Briggs, R. C. and Hnilica, L. S., Science, 211, 70 (1981).
5.
Glass, W. F., Briggs, R. C. and Hnilica, L. S., Anal. Biochem., 115, 219
(1981).
6.
Karcher, D., Lowenthal, A., Thormar, H. and Noppe, M., J. Immunol.
Meth., 43, 175 (1981).
7.
Reiser, J. and Wardale, J., Eur. J. Biochem., 114, 569 (1981).
8.
Olmstead, J. B., J. Biol. Chem., 256, 11955 (1981).
9.
Symington, J. M., Green, J. and Brackmann, K., PNAS, 78, 177 (1981).
10. Burnette, W. N., Anal. Biochem., 112, 195 (1981).
11. Legocki, R. P. and Verma, D. S. P., Anal. Biochem., 111, 385 (1981).
12. Erlich, H. A., Levinson, J. R., Cohen, S. N. and McDevitt, M. O., J. Biol.
Chem., 254, 12240 (1979).
13. Akerstrom, B. and Bjorck, L., J. Biol. Chem., 261, 10240 (1986).
21
Problem
Probable cause
Recommended solution
Blot is washed
Tween-20 is necessary in the
in the absence
washes to reduce back
of Tween-20.
ground.
Conjugate is
Use the recommended
used at an ex-
1:3,000 dilution.
cessive con-
centration.
Contamination
Refer to the Trans-Blot,
occurred dur-
Trans-Blot SD, or Mini
ing transfer.
Trans-Blot manual.
Use of poor
Use pure nitrocellulose from
quality, mixed
Bio-Rad.
ester nitrocel-
lulose will cause
increased back-
ground.
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