Bio-Rad HRP and AP Conjugates - Manual (Page 2)

Specifications
Contents 2 ml
Buffer 10 mM sodium phosphate,
150 mM sodium chloride,
pH 7.2. Contains 1.0%
bovine serum albumin and
0.01% thimerosal.
Preparation Avidin is conjugated to horseradish
of conjugate peroxidase using a modification
of the periodate oxidation procedure
described by Wilson and Nakane
(1978).
Recommended 1:1,000 when used in the
working dilution Biotin-Blot ™ protein detection
system; 1:3,000 when used
with biotinylated SDS-PAGE
standards. Different dilutions
may be optimal for other
applications.
Shelf life 1 year at 4ºC from date thawed
3 years at –20ºC from date of
manufacture
2
LIT423E.qxd 8/4/2004 2:13 PM Page 2