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Specifications – Bio-Rad HRP and AP Conjugates User Manual

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Specifications

Contents

2 ml

Buffer

10 mM sodium phosphate,
150 mM sodium chloride,
pH 7.2. Contains 1.0%
bovine serum albumin and
0.01% thimerosal.

Preparation

Avidin is conjugated to horseradish

of conjugate

peroxidase using a modification
of the periodate oxidation procedure
described by Wilson and Nakane
(1978).

Recommended

1:1,000 when used in the

working dilution

Biotin-Blot

protein detection

system; 1:3,000 when used
with biotinylated SDS-PAGE
standards. Different dilutions
may be optimal for other
applications.

Shelf life

1 year at 4ºC from date thawed
3 years at –20ºC from date of
manufacture

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