Specifications – Bio-Rad HRP and AP Conjugates User Manual
Page 2

Specifications
Contents
2 ml
Buffer
10 mM sodium phosphate,
150 mM sodium chloride,
pH 7.2. Contains 1.0%
bovine serum albumin and
0.01% thimerosal.
Preparation
Avidin is conjugated to horseradish
of conjugate
peroxidase using a modification
of the periodate oxidation procedure
described by Wilson and Nakane
(1978).
Recommended
1:1,000 when used in the
working dilution
Biotin-Blot
™
protein detection
system; 1:3,000 when used
with biotinylated SDS-PAGE
standards. Different dilutions
may be optimal for other
applications.
Shelf life
1 year at 4ºC from date thawed
3 years at –20ºC from date of
manufacture
2
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