Bio-Rad Sequi-Blot™ PVDF Membrane User Manual
Page 11

Section 7
For Technical Assistance
In the U.S., technical service is available by
calling 1-800-4BIORAD (1-800-424-6723).
Our Technical Service representatives are
available to answer your questions from 8 AM
to 5 PM (PST). Technical representatives can
be contacted through your local Bio-Rad office.
For more information about the use of PVDF
membrane, request bulletin 2212.
17
Section 6
Protein Detection
Staining
Proteins for sequencing can be detected
with Coomassie brilliant blue (CBB) R-250 or
with Bio-Rad’s Colloidal Gold Total Protein
Stain. CBB R-250 is the stain most commonly
used for detection of proteins prior to protein
sequence analysis. It is rapid (² 15–20 minutes),
sensitive, and does not interfere or react with
the reagents used in Edman degradation chem-
istry. See page 13 for the CBB R-250 stain
preparation without acetic acid. If a protein can
not be detected by CBB staining, there will not
be enough present to sequence. See the Blotting
for Protein Sequencing section for a protocol
for CBB R-250 staining.
16
LIT240c.qxd 6/23/98 11:42 AM Page 16
- TransFectin™ Lipid Reagent (2 pages)
- Gene Pulser MXcell™ Electroporation System (19 pages)
- Gene Pulser MXcell™ Electroporation System (66 pages)
- Gene Pulser Xcell™ Electroporation Systems (83 pages)
- Gene Pulser® Electroporation Buffer (2 pages)
- MicroPulser™ Electroporator (31 pages)
- Helios® Gene Gun System (52 pages)
- PDS-1000 / He™ and Hepta™ Systems (51 pages)
- TGX™ FastCast™ Acrylamide Solutions (2 pages)
- Criterion™ TGX Stain-Free™ Precast Gels (16 pages)
- Criterion Stain Free™ Tris-HCl Gels (96 pages)
- Image Lab™ Software (212 pages)
- Gel Doc™ EZ System (22 pages)
- Mini-PROTEAN® TGX™ Precast Gels (52 pages)
- ChemiDoc™ MP System (8 pages)
- Image Lab™ Software (236 pages)
- ChemiDoc™ XRS+ System (42 pages)
- ChemiDoc™ XRS+ System (4 pages)
- ChemiDoc™ XRS+ System (50 pages)
- GS-800™ Calibrated Densitometer (444 pages)
- Criterion™ TGX™ Precast Gels (60 pages)
- Criterion™ Cell (13 pages)
- Image Lab™ Software (260 pages)
- Criterion™ XT Bis-Tris Precast Gels (26 pages)
- 2-D Electrophoresis Workflow (14 pages)
- ReadyPrep™ 2-D Starter Kit (28 pages)
- EXQuest Spot Cutter (81 pages)
- Fluorescent Protein Stains (27 pages)
- 2-D Electrophoresis Workflow (22 pages)
- 2-D Electrophoresis Workflow (20 pages)
- PROTEAN® i12™ IEF System (4 pages)
- Ready Gel® Zymogram Precast Gels (46 pages)
- Mini-PROTEAN 2-D Electrophoresis Cell (44 pages)
- Precision Plus Protein™ Prestained Standards (3 pages)
- Precision Plus Protein™ Unstained Standards (16 pages)
- Prestained SDS-PAGE Standards (3 pages)
- Unstained SDS-PAGE Standards (3 pages)
- Silver Stains (20 pages)
- Biotinylated Standards (3 pages)
- Biotinylated Standards (11 pages)
- IEF and 2-D Standards (3 pages)
- Mini-PROTEAN 2-D Electrophoresis Cell (20 pages)
- Mini-PROTEAN® Tetra Handcast Systems (10 pages)
- Mini-PROTEAN 3 Multi-Casting Chamber (10 pages)
- PROTEAN® Plus Hinged Spacer Plates and Combs (21 pages)