Bio-Rad Immun-Blot® PVDF Membrane - Manual (Page 3)

Section 1
Introduction
PVDF (polyvinylidene difluoride) mem-
brane was originally introduced to protein use as
an ideal medium for the harsh chemical environ-
ment of N-terminal, or Edman degradation,
sequencing. The hydrophobicity of PVDF
makes it an ideal support for binding proteins in
electrophoretic and dot blotting applications.
Because of the hydrophobic nature of PVDF, it
does require a prewetting step in alcohol. The
high protein binding capacity, target retention,
and resistance to cracking has made PVDF an
appealing membrane for general lab techniques.
The two main applications for PVDF are N-
Terminal Sequencing and Immunoblotting, both
of which benefit from the qualities of PVDF but
in different aspects of the membrane. While
sequencing work is concerned with retaining as
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