Bio-Rad Trans-Blot® SD Semi-Dry Transfer Cell - Manual (Page 9)

Section 6
Appendix
10x TBE (per liter*), pH 8.3
Quantity Final 0.5x Concentration
108 g Tris base 44.5 mM Tris base
55 g boric acid 44.5 mM boric acid
40 ml 0.5 M EDTA, pH 8.0 1 mM EDTA
To make a 0.5x TBE working buffer, mix 50 ml of the 10x TBE stock per 1 liter of deion-
ized, distilled water.
* One liter of Bio-Rad premixed 10x TBE is included with the kit.
1x MOPS, 1 L
Quantity Final 1x Concentration
4.186 g MOPS 20 mM 3- (N- Morpholino) propanesulfonic acid, pH 7.0
0.41 g sodium acetate 5 mM sodium acetate
2 µl of 0.5 M EDTA, pH 8.0 1 mM EDTA
Dissolve 4.186 g MOPS, 0.41 g sodium acetate, and 2 µl EDTA in 800 ml of ddH
2
0;
adjust volume to l liter with ddH
2
0.
37% formaldehyde stock solution
1.8% formaldehyde - 5 ml of 37% stock/ 100 ml
6.5% formaldehyde - 1.75 µl of 37% stock/ 10 µl
20x SSC, 1 L
Quantity Final 20x Concentration
175.3 g NaCl 3 M NaCl
88.2 g sodium citrate 0.3 M sodium citrate
Adjust pH to 7.0 and bring volume to 1 liter with deionized, distilled water. Dilute the 20x
stock solution accordingly to make a 2x working solution.
Gel loading buffer - Bromophenol blue dye
1mM EDTA, pH 8.8
0.25% bromophenol blue
0.25% xylene cyanol
50% glycerol
6