Bio-Rad Acrylamide Solutions and Powders - Manual

Initiators
Chemical polymerization is initiated by
Ammonium Persulfate and TEMED. They are reactive
by nature and may be prone to oxidation or decomposi-
tion. APS can be stored desiccated at room temperature
for at least 1 year. Solutions should be made fresh daily,
since persulfate in solution decomposes rapidly.
TEMED can be stored tightly closed and desiccated
either at 4 °C or at room temperature for at least 6
months. After 10 to 12 months, there is a significant
reduction in activity and an increase in the amount
required for polymerization. If refrigerated, TEMED
should be warmed to room temperature before opening
to avoid the release of condensation into solution.
Temperature and Oxygen
Oxygen is an inhibitor of free radical polymeriza-
tion. Proper degassing of the gel solutions immediately
prior to pouring the gel is therefore critical for repro-
ducibility. To insure faster and more complete
degassing, bring the gel solution to room temperature
first, as cold solutions have a greater capacity for dis-
solved oxygen. Pouring a gel with a cold solution will
have a substantial negative effect on the rate of polymer-
ization and on the quality of the resulting gel as well.
Contaminants in Buffers
Contaminants in buffer reagents, gel additives, and
laboratory water can inhibit polymerization. The most
common contaminants of these reagents are metals,
non-ion buffers, and breakdown products. Partially
inhibited polymerization will result in gels with greater
porosity than intended, and the molecules will have
greater mobilities.
For a more detailed discussion of gel polymeriza-
tion, request bulletin 1156 from your Bio-Rad
Representative, or in the U.S. call 1-800-4BIORAD.
4
Section 5
Risk and Safety Information
Hazardous Acrylamide
components N-N’-Methylene-bis-acrylamide
Risk and May cause cancer. May cause heritable
safety phrases genetic damage. Toxic in contact with skin
and if swallowed. Danger of serious dam-
age to health by prolonged exposure. Avoid
exposure - obtain special instructions before
use. If you feel unwell, seek medical advice
and show label where possible.
Kann Krebs erzeugen. Kann vererbbare
Schäden verursachen. Giftig bei Berührung
mit der Haut und beim Verschlucken. Bei
längerer Exposition Gefahr ernster Gesund-
heitsschäden. Exposition vermeiden - vor
Gebrauch besondere Anweisungen ein-
holen. Bei Unwohlsein ärztlichen Rat ein-
holen. Wenn möglich dieses Etikett
vorzeigen.
Peut causer le cancer. Peut causer des
altérations génétiques héréditares. Toxique
par contact avec la peau et par ingestion.
Risque d´effets graves pour la santé en cas
d´exposition prolongée. Eviter l´exposition -
se procurer des instructions spéciales avant
l´utilisation. En cas de malaise consulter un
médecin (si possible lui montrer l´étiquette).
Può provocare il cancro. Può provocare
alterazioni genetiche ereditarie. Tossico a
contatto con la pelle e per ingestione. Peri-
colo di gravi danni per la salute in caso di
esposizione prolungata. Evitare l´espo-
sizione - procurarsi speciali istruzioni prima
dell´uso. In caso di malessere consultare il
medico (se possibile, mostrargli l´etichetta).
Safety symbol h
CAS number 79-06-1 (Acrylamide)
110-26-9 (N-N’-Methylene-bis-acrylamide)
RID/ADR number 6.1/12c UN 2074
RTECS number AS 3325000
EINECS number 2011737
WGK number 3
Bio-Rad Laboratories, 2000 Alfred Nobel Drive, Hercules, CA 94547
LIT306 Rev C
Premixed Preweighed
Acrylamide/Bis
19:1, 30 g Catalog Number 161-0120
19:1, 150 g Catalog Number 161-0123
29:1, 30 g Catalog Number 161-0121
29:1, 150 g Catalog Number 161-0124
37.5:1, 30 g Catalog Number 161-0122
37.5:1, 150 g Catalog Number 161-0125
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