Bio-Rad PROTEAN Plus Multi-Casting Chamber User Manual
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Section 1
General information
1.1 Introduction
The PROTEAN plus Multi-Casting Chamber allows four to twelve 1.0, 1.5 or 2.0 mm gels
to be cast simultaneously for use in the PROTEAN plus Dodeca Cell. Monomer can be
introduced from the top or the bottom to prepare identical linear gradient or single percentage
gels. Cast gels can be stored up to 1 week at 4 ºC.
The PROTEAN plus Multi-Casting Chamber is not used to cast gels for the PROTEAN
II Cell. For this application, use the PROTEAN II xi Multi-Gel Casting Chamber, catalog
number 165-2025.
1.2 Specifications
Construction
Casting chamber
fabricated acrylic
Sealing plate
fabricated acrylic with captive, spring-loaded
screws
Gasket
silicone tubing
Weight
3.9 kg
Approximate size
32 cm x 23 cm x 12 cm
Gel size
20 x 20.5 (W x L) cm and 25 x 20.5 (W x L) cm in
1.0 mm, 1.5 mm, 2.0 mm thicknesses
PROTEAN plus hinged
26.8 x 22.5 cm (W x L)
spacer plate size
Note: PROTEAN plus multi-casting chamber components are not compatible with chlorinated
hydrocarbons (e.g. chloroform), aromatic hydrocarbons (e.g. toluene, benzene), acetone,
glacial acetic acid, carbon tetrachloride, chromic acid (40%), diethyl ether, dimethyl
formamide, ethyl acetate, xylene, and ethanol. Use of such solvents voids all warranties. To
insure best performance of the multi-casting chamber, become fully acquainted with these
instructions before use. All components should be cleaned with a suitable laboratory detergent
(Bio-Rad’s Cleaning Concentrate, catalog number 161-0772), rinsed thoroughly with
distilled water, and dried before use.
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