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Bio-Rad Mini-PROTEAN 3 Multi-Casting Chamber User Manual

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Table of Contents

Page

Section 1

General Information....................................................................................1

1.1 Introduction ................................................................................................................1
1.2 Specifications .............................................................................................................1

Section 2

Loading the Chamber..................................................................................1

Section 3

Casting Gels ..................................................................................................2

3.1

Casting Non-Gradient Gels from the Top .................................................................2

3.2

Casting Linear and Convex Gradient Gels from the Bottom ....................................3

Section 4

Chamber Disassembly and Storage of Gels ..............................................3

Section 5

Using the Gels ...............................................................................................4

Section 6

Ordering Information..................................................................................4

Section 7

Reagents for Electrophoresis ......................................................................5

Appendix A Estimated Volume of Acrylamide for 12 Mini-PROTEAN 3 Gels .........6

Appendix B Guidelines for Gel Casting ..........................................................................7

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