Bio-Rad Biotinylated Standards - Manual (Page 2)

Table of Contents
Section 1 Introduction .......................................................................... 1
Section 2 Specifications ........................................................................ 3
Section 3 Safety Instructions................................................................ 4
Section 4 Solutions ................................................................................ 5
4.1 Solutions for Electrophoresis Gels ......................................... 5
4.2 Solutions for Nitrocellulose Membranes................................ 5
Section 5 Assay Procedure ................................................................... 7
5.1 General Recommendations..................................................... 7
5.2 Protocol for SDS-PAGE Electrophoresis ............................... 7
5.3 Protocol for Immune Detection .............................................. 8
Section 6 Troubleshooting Guide ........................................................ 9
6.1 Activity Test for Reagents...................................................... 9
6.2 No Reaction or Weak Color Development............................. 9
6.3 No Color in Standards, Good Color in Sample ...................... 10
6.4 No Color in Sample, Good Color in Standards ...................... 11
6.5 High Background.................................................................... 11
Section 7 References ............................................................................. 12
Section 8 Ordering Information .......................................................... 13
4006050C 5/24/99 8:35 AM Page i