Bio-Rad Precision Plus Protein™ Standard Plugs - Manual (Page 7)

5
Instructions
Precision Plus Protein Standard Plugs are ready-to-
use 2-D electrophoresis standards. When ready to
use Precision Plus Protein standard plugs, break off
one of the ends of the plastic plug casing. For each
application, break off an individual plastic plug by
placing pressure on the flat side to break the
junction, then twisting the individual plug off.** Then
break off the attached spatula. Prepare SDS-PAGE
gels by blotting away any excess water remaining
inside the IPG well using blotting paper. Then use
the spatula to slide the plug outward and onto the
top plate of your gel. Use the spatula to push the
plug into the well next to the IPG strip. See Figures
1-8 for detailed illustrations of how to use the
Precision Plus Protein standard plugs.
The Precision Plus Protein standard plugs
have been optimized for 12% PROTEAN Plus
gels. If you are using a gel of a
different formulation, add 5% BME or 50 mM
DTT to your overlay agarose.
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