Bio-Rad iTaq™ DNA Polymerase - Manual (Page 2)

Typical Thermal Cycling Protocol
C Cy yc clle e 1 1 3 min, 95ºC
Polymerase activation
C Cy yc clle e 2 2: 25–40 repeats 30 sec, 95ºC
30 sec, 55ºC
30 sec – X min, 72ºC (depending on length of PCR product)
C Cy yc clle e 3 3 Hold at 4ºC
Reagents and Materials Not Supplied
10 mM dNTP mix (Catalog # 170-8874)
Microcentrifuge tubes, screw capped
Pipette tips, preferably aerosol barrier tips
Vortexer
Microcentrifuge
Tubes for thermal cycler
Sterile water
Primers
iCycler
® thermal cycler
Recommendations for Best Results
Due to the sensitivity of PCR, precautions should always be taken to minimize contamination.
Steps at all stages of setting up reactions should be designed to prevent reagent contamination
with samples, primers, and previous reaction products. This should involve the use of separate
areas for reaction set up, template preparation, and reaction product analysis. Also the use of
barrier pipet tips and non-flip cap tubes for templates, dilutions, and primers are recommended for
reducing contamination.
To learn more about Bio-Rad’s complete solution for amplification, visit our website:
www.bio-rad.com/amplification
NOTICE TO PURCHASER: LIMITED LICENSE
Use of this product is covered by one or more of the following US patents and corresponding patent claims
outside the US: 5,079,352, 5,789,224, 5,618,711, 6,127,155 and claims outside the US corresponding to
US Patent No. 4,889,818. The purchase of this product includes a limited, non-transferable immunity from
suit under the foregoing patent claims for using only this amount of product for the purchaser’s own internal
research. No right under any other patent claim (such as the patented 5’ Nuclease Process claims in the US
Patent Nos. 5,210,015 and 5,487,972), no right to perform any patented method, and no right to perform
commercial services of any kind, including without limitation reporting the results of purchaser’s activities for
a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel. This product
is for research use only. Diagnostic uses under Roche patents require a separate license from Roche. Further
information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied
Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.
4106202 Rev B
Bio-Rad Laboratories, Inc.
2000 Alfred Nobel Drive, Hercules, CA 94547
510-741-1000
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