Bio-Rad iScript™ Reverse Transcription Supermix for RT-qPCR - Manual (Page 2)

iScript ™ Reverse Transcription Supermix for RT-qPCR
Bio-Rad Laboratories, Inc.
2000 Alfred Nobel Drive, Hercules, CA 94547
510-741-1000 10020178 Rev B 13-1710 1113
Recommendations for the Use of No-RT Control
■ ■
Interference of gene expression analysis by genomic DNA
carryover in RNA samples can be tested using the no-RT
control supermix
■ ■
Setting up a no-RT control reaction with the same amount
of total RNA as the reverse transcription reaction is
recommended to allow similar carryover of cDNA synthesis
components in qPCR for accurate detection of genomic
DNA amplicons
Recommendations for qPCR
■ ■
cDNA generated with this kit can be used directly in qPCR
■ ■
The volume of cDNA synthesis reaction used must not
exceed 10% of the qPCR volume
■ ■
cDNA can be diluted in 10 mM Tris-HCl (pH 8.0), 0.1 mM
EDTA prior to use in qPCR. The optimum cDNA dilution
must be determined based on target gene abundance and
qPCR chemistry
Related Products
Reverse transcription reagents for real-time qPCR:
■ ■
iScript advanced cDNA synthesis kit for RT-qPCR (170-8842)
■ ■
iScript cDNA synthesis kit (170-8890)
Reagents for real-time qPCR:
■ ■
SsoAdvanced ™ universal SYBR ® Green supermix (172-5270)
■ ■
SsoAdvanced universal probes supermix (172-5280)
■ ■
iTaq ™ universal SYBR ® Green supermix (172-5120)
■ ■
iTaq universal probes supermix (172-5130)
To learn more about Bio-Rad’s complete solution for
amplification, visit www.bio-rad.com/amplification.
SYBR is a trademark of Life Technologies Corporation. Bio-Rad Laboratories,
Inc. is licensed by Life Technologies Corporation to sell reagents containing
SYBR Green I for use in real-time PCR, for research purposes only.
Practice of the patented 5' Nuclease Process requires a license from Applied
Biosystems. The purchase of iTaq and SsoAdvanced supermixes includes an
immunity from suit under patents specified in the product insert to use only the
amount purchased for the purchaser's own internal research when used with
the separate purchase of Licensed Probe. No other patent rights are conveyed
expressly, by implication, or by estoppel. Further information on purchasing
licenses may be obtained from the Director of Licensing, Applied Biosystems,
850 Lincoln Centre Drive, Foster City, California 94404, USA.