LaMotte NITROGEN MODULE AM-31 - Manual (Page 2)

PRO CE DURE
1.
Fill a test tube (0755) to line 7 with *Universal Extracting Solution
(5173).
2. Use 0.5 g spoon (0698) to add four level measures of soil. Cap and
shake for one minute.
NOTE:
When adding samples with high concentrations of
carbonates to *Universal Extracting Solution (5173), swirl tube to
mix for 30 seconds before capping to allow gases to escape.
3. Fold a piece of filter paper (0465) in half. Fold in half again. Gently
push corners together to form a cone. Place cone in funnel (0459).
4. Place funnel in clean filtrate tube (0749) and filter suspension
through the filter paper. The clear solution is the extract.
5. Use the 1 mL pipet (0354) to transfer 1 mL of soil extract to one of
the depressions on the spot plate (0159).
5. Use pipet (0392) to add 10 drops of *Nitrate Reagent #1 (5146).
6. Use the 0.5g spoon (0698) to add one measure of *Nitrate Reagent
#2 (5147). Stir thoroughly with plastic rod. Wait five minutes.
7. Match sample color to a color standard on Nitrate-Nitrogen Color
Chart (1315). Record as lb/acre nitrate-nitrogen.
LaMOTTE COM PANY
Help ing Peo ple Solve An a lyt i cal Chal lenges ®
PO Box 329 • Chestertown • Mary land • 21620 • USA
800-344-3100 • 410-778-3100 (Out side U.S.A.) • Fax 410-778-6394
Visit us on the web at www.lamotte.com
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