Lamotte com pany – LaMotte ALGAE IN WATER TEST KIT AWL User Manual
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2.
Insert the end of the check valve (27760-01), that does not have threads and
does have small tabs, into the Luer tip of the syringe (0943).
3.
Insert the opposite end of the check valve with the threads into the
larger opening in the filter holder.
4.
Depress the syringe plunger to the 0 position. Attach the plastic tubing
(1175) to the side arm of the check valve. Submerge the free end of the
tubing in the sample water.
PRO CE DURE
1.
After the syringe-filter device has been prepared according to above
procedure, complete one stroke of the syringe by pulling the plunger out
and drawing water into the syringe through the plastic tubing. Fill the
barrel to the 50 mL mark. Slowly depress the plunger to expel the water
through the filter holder. Repeat at least 4 times.
NOTE:
When excessive pressure is exerted upon the filter disc by
depressing the plunger too rapidly, there is a tendency for the filter disc to
rupture. If this happens, replace filter disc and repeat sampling procedure.
2.
Disconnect filter holder from the syringe. Unscrew the filter holder and
carefully remove the filter disc. The presence of a green-yellow color on the
filter disc indicates algae may be present.
3.
Place the filter disc in the test tube (0608). Fill to 5 mL line with
*Methyl Alcohol (6319). Cap and shake vigorously for approximately 2
minutes. This will extract the green chlorophyll from the algae cells and
disintegrate the disc. The disintegrated disc must be filtered out of the
solution.
4.
Place a new filter disc in the filter holder.
5.
Remove the check valve from syringe. Attach the filter holder directly to
syringe.
6.
Remove the plunger from the syringe and pour the contents of the test tube
into the syringe barrel. Place the outlet of the filter holder into the test tube
(0230), replace the plunger in the syringe, and slowly depress until all of the
solution has been collected in the test tube.
7.
View across the diameter of the test tube or down through the solution.
A clear yellow to green color indicates algae is present.
LaMOTTE COM PANY
Helping Peo ple Solve An a lyt i cal Challenges
SM
PO Box 329 • Chestertown • Mary land • 21620 • USA
800-344-3100 • 410-778-3100 (Out side U.S.A.) • Fax 410-778-6394
Visit us on the web at www.lamotte.com
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