LaMotte ALGAE IN WATER KIT - Manual (Page 2)

FILTRATION ASSEMBLY
1. Unscrew the cover of the fi lter holder (0598) and install a fi lter paper (1157) in the
holder. Position the paper carefully to avoid by-passing the fi lter. If a membrane
fi lter is used, install a support pad in back of the membrane disc. Replace the cover.
2. Insert the end of the check valve (27760-01), that does not have threads and does
have small tabs, into the Luer tip of the syringe (0943).
3. Insert the opposite end of the check valve with the threads into the larger opening
in the fi lter holder.
4. Depress the syringe plunger to the 0 position. Attach the plastic tubing (1175) to
the side arm of the check valve. Submerge the free end of the tubing in the sample
water.
PROCEDURE
1. After the syringe-fi lter device has been prepared according to above procedure,
complete one stroke of the syringe by pulling the plunger out and drawing water
into the syringe through the plastic tubing. Fill the barrel to the 50 mL mark.
Slowly depress the plunger to expel the water through the fi lter holder. Repeat at
least 4 times.
NOTE: When excessive pressure is exerted upon the fi lter disc by depressing
the plunger too rapidly, there is a tendency for the fi lter disc to rupture. If this
happens, replace fi lter disc and repeat sampling procedure.
2. Disconnect fi lter holder from the syringe. Unscrew the fi lter holder and carefully
remove the fi lter disc. The presence of a green-yellow color on the fi lter disc
indicates algae may be present.
3. Place the fi lter disc in the test tube (0608). Fill to 5 mL line with *Methyl
Alcohol (6319). Cap and shake vigorously for approximately 2 minutes. This will
extract the green chlorophyll from the algae cells and disintegrate the disc. The
disintegrated disc must be fi ltered out of the solution.
4. Place a new fi lter disc in the fi lter holder.
5. Remove the check valve from syringe. Attach the fi lter holder directly to syringe.
6. Remove the plunger from the syringe and pour the contents of the test tube into
the syringe barrel. Place the outlet of the fi lter holder into the test tube (0230),
replace the plunger in the syringe, and slowly depress until all of the solution has
been collected in the test tube.
7. View across the diameter of the test tube or down through the solution. A clear
yellow to green color indicates algae is present.
LaMOTTE COMPANY
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PO Box 329 • Chestertown • Maryland • 21620 • USA
800-344-3100 • 410-778-3100 (Outside U.S.A.) • Fax 410-778-6394
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