LaMotte NITROGEN IN SOIL TEST KIT - Manual (Page 2)

PRO CE DURE
SOIL SAM PLING
Carefully follow the instructions given in the LaMotte Soil Handbook (1504)
to prepare the soil sample.
EX TRAC TION
1. Use the 1 mL pipet (0354) to add 1 mL of *Acid Extracting
Solution (6361) to a test tube (0701). Dilute to the 15 mL line with
deionized water.
2. Use 1.0g spoon (0697) to add three measures of soil. Use 0.5 mL
pipet (0369) to add 0.5 mL of Charcoal Suspension (5638). Cap and
shake for 5 minutes.
3. Fold filter paper in half then in half again to form a triangle. Squeeze
corners together to open filter paper and form a cone. Insert into
funnel (0459).
4. Filter sample into a second graduated vial. This liquid will be used as
the sample in the test.
NI TRO GEN TEST
1. Fill a test tube (0755) to the 3 mL line with filtered soil extract.
2. Dilute to 6 mL line with *Mixed Acid Reagent (V-6278). Cap and
mix.
3. Use the 0.1g spoon (0699) to add one measure of *Nitrate Reducing
Reagent (V-6279). Cap and mix.
4. Mix by inverting approximately 50-60 times in a period of one
minute. Wait 10 minutes.
NOTE:
At the end of the waiting period an undissolved portion of
the *Nitrate Reducing Reagent (V-6279) may remain in the bottom
of the tube without affecting the results.
5. Holding tube ½” above white area of Nitrogen Color Chart (1371),
match sample color to a color standard. Use chart to convert result
to a reading. Record as lb/A Nitrogen.
lb/A
Trace
10
Low
20
Medium
40
High
100
LaMOTTE COM PANY
Helping Peo ple Solve An a lyt i cal Challenges SM
PO Box 329 • Chestertown • Mary land • 21620 • USA
800-344-3100 • 410-778-3100 (Out side U.S.A.) • Fax 410-778-6394
Visit us on the web at www.lamotte.com
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