LaMotte TRACE ELEMENT PLANT TISSUE TESTS PT-04 - Manual (Page 3)

The tests described below largely follow the methods described by Dr. S.R.
Melstead of the University of Illinois.
MAN GA NESE
1.
Use plain filter paper (0471) to collect plant sap.
2. Use the glass pipet (0341) to add 1 drop of *Manganese Reagent #1
(5262) to area containing sap. Wait 30 seconds.
3. Use plain pipet (0342) to add one drop of *Manganese Reagent #2
(5263). Wait one minute.
4. The area will turn blue if sufficient manganese is present.
IRON - FER ROUS AND FER RIC
1.
Use plain filter paper (0471) to collect plant sap.
2. Use the plastic pipet (0357) to add one drop of *Ferrous Iron Reagent
(5264) to a spot of sap.
3. Add one drop of *Ferrous & Ferric Iron Reagent (5265) to a second
spot of sap.
4. The area(s) will turn red if sufficient iron is present.
ZINC
1.
Use plain filter paper (0471) to collect the plant sap.
2. Prepare Zinc Reagent in the following manner:
a. Use the 1.0 mL pipet (0354) to transfer 1 mL of Deionized Water
(5115PS) to a test tube (0231).
b. Use the 0.5 g spoon (0698) to add 0.5 g of *Zinc Reagent Powder
(7393). Mix. The mixture will contain some undisolved material. It
should be discarded at the end of the day.
3. Add one drop of the prepared reagent to the area on the filter paper
containing a spot of sap. Wait 2-3 minutes.
4. The area will turn blue if sufficient zinc is present.