LaMotte TRACE ELEMENT PLANT TISSUE KIT SAP SPOT TEST - Manual (Page 3)

The tests described below largely follow the methods described by Dr. S.R. Melstead of
the University of Illinois.
MANGANESE
1. Use plain fi lter paper (0471) to collect plant sap.
2. Use the glass pipet (0341) to add 1 drop of *Manganese Reagent #1 (5262) to area
containing sap. Wait 30 seconds.
3. Use plain pipet (0342) to add one drop of *Manganese Reagent #2 (5263). Wait
one minute.
4. The area will turn blue if suffi cient manganese is present.
IRON - FERROUS AND FERRIC
1. Use plain fi lter paper (0471) to collect plant sap.
2. Add one drop of *Ferrous Iron Reagent (5264) to a spot of sap.
3. Use the plastic pipet (0357) to add one drop of *Ferrous & Ferric Iron Reagent
(5265) to a second spot of sap.
4. The area(s) will turn red if suffi cient iron is present.
ZINC
1. Use plain fi lter paper (0471) to collect the plant sap.
2. Prepare Zinc Reagent in the following manner:
3. Use the 1.0 mL pipet (0354) to transfer 1 mL of Deionized Water (5115PS) to a
test tube (0231).
4. Use the 0.5 g spoon (0698) to add 0.5 g of *Zinc Reagent Powder (7393). Mix.
The mixture will contain some undisolved material. It should be discarded at the
end of the day.
5. Add one drop of the prepared reagent to the area on the fi lter paper containing a
spot of sap. Wait 2-3 minutes.
6. The area will turn blue if suffi cient zinc is present.
COPPER
1. Use a Copper Test Paper (5266) to collect the plant sap.
2. Add 1 drop of *Copper Test Solution (5267) to an area of the paper containing a
spot of plant sap.
3. The area will turn blue if suffi cient copper is present.
BORON
1. Use a Boron Test Paper (5268) to collect the plant sap.
2. Outline a spot of the plant sap with a pencil and mark a spot of similar size on
another strip of test paper. Add one drop of Deionized Water (5115PS) to second
strip.
3. After a few minutes, the area which has the plant sap should turn a bluish to
a purplish color. No color should appear in the deionized water blank. If a
difference of color exists, the plant contains suffi cient boron.