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LaMotte AQ-5 Saltwater Aquaculture Kit User Manual

Salt water aquaculture test kit

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SALT WATER AQUACULTURE

TEST KIT

MODEL AQ-5 · CODE 3636-03

NOTE: It is important to read the instruction manual
before attempting to perform the tests with the short
form instructions provided below.
*WARNING:

Reagents marked with a * are

considered to be potential health hazards. To view or
print a Material Safety Data Sheet (MSDS) for these
reagents see MSDS CD or www.lamotte.com. To obtain
a printed copy, contact LaMotte by e-mail, phone or
fax.
To order individual reagents or test kit components, use
the specified code number.

AM MO NIA NI TRO GEN

1.

Fill test tube (0106) to 5 mL line with sample water.

2.

Add 10 drops *Salicylate Ammonia #1 (3978LWT).

Cap and mix.

3.

Add 7 drops of *Salicylate Ammonia #2 (3979WT).

Cap and mix. Wait 1 minute.

4.

Add 7 drops of Salicylate Ammonia #3 (3982WT). Cap

and mix. Wait 20 minutes.

5.

Insert test tube into Octa-Slide Viewer (1100) with

Ammonia Nitrogen Octa-Slide Bar (3438) inserted.

6.

Record as ppm Ammonia Nitrogen (NH

3

-N).

NI TRITE NI TRO GEN

1.

Fill test tube (0106) to 2.5 mL line with sample water.

2.

Dilute to 5 mL line with *Mixed Acid Reagent

(V-6278).

3.

Use 0.1g spoon (0699) to add 0.1g of *Color

Developing Reagent (V-6281). Cap and mix for 1

minute. Wait 5 minutes.

4.

Insert test tube into Octa-Slide Viewer (1100) with

Nitrite Nitrogen Octa-Slide Bar (3437) inserted.

5.

Record as ppm Nitrite Nitrogen (NO

2

-N).

NI TRATE NI TRO GEN

1.

Fill test tube (0106) to 2.5 mL line with sample water.

2.

Dilute to 5 mL line with *Mixed Acid Reagent

(V-6278). Cap and mix. Wait 2 minutes.

3.

Use 0.1g spoon (0699) to add one level measure of

*Nitrate Reducing Reagent (V-6279). Cap and invert

tube 50-60 times in one minute. Wait 10 minutes.

4.

Invert sample one time and insert test tube into

Octa-Slide Viewer (1100) with Nitrate Nitrogen

Octa-Slide Bar (3413) inserted.

5.

Record as ppm Nitrate Nitrogen (NO

3

-N).

AL KA LIN ITY

1.

Fill titration tube (0608) to 5 mL line with sample

water.

2.

Add 4 drops of *BCG-MR Indicator (2311-EG). Cap

and mix. Sample will turn blue-green.

3.

Fill Direct Reading Titrator (0382) with *Alkalinity

Titration Reagent B (4493DR).

4.

Titrate sample until blue-green color changes to pink.

5.

Record as ppm Alkalinity (CaCO

3

).

CARBON DI OX IDE

1.

Fill titration tube (0608) to 20 mL line with sample

water.

2.

Add 2 drops *Phenolphthalein Indicator, 1% (2246). If

sample turns red, no free carbon dioxide is present. If

colorless, proceed to Step 3.

3.

Fill Direct Reading Titrator (0380) with *Carbon

Dioxide Reagent B (4253DR).

4.

Titrate sample until faint pink color persists for 30

seconds.

5.

Record as ppm Carbon Dioxide (CO

2

).

LaMOTTE COM PANY

Helping Peo ple Solve An a lyt i cal Challenges

SM

PO Box 329 · Chestertown · Mary land · 21620 · USA

800-344-3100 · 410-778-3100 (Out side U.S.A.) · Fax 410-778-6394 · www.lamotte.com

3636-03-SF · 02/09