LaMotte AQ-2 Freshwater Aquaculture Kit - Manual

FRESH WATER AQUACULTURE
TEST KIT
MODEL AQ-3 · CODE 3634-03
NOTE: Place dispenser Cap (0692) on *Mixed Acid
Reagent (V-6278). Save this cap for refills. It is important
to read the instruction manual before attempting to
perform the tests with the short form instructions provided
below.
*WARNING:
Reagents marked with a * are considered to
be potential health hazards. To view or print a Material
Safety Data Sheet (MSDS) for these reagents see MSDS
CD or www.lamotte.com. To obtain a printed copy, contact
LaMotte by e-mail, phone or fax.
To or der in di vid ual re agents or test kit com po nents, use the
spec i fied code num ber.
AMMONIA NITROGEN
1. Fill test tube (0106) to 5 mL line with sample water.
2. Add 4 drops Ammonia Nitrogen Reagent #1
(4797WT). Cap and mix. Wait 1 minute.
3. Add 12 drops of *Ammonia Nitrogen Reagent #2
(4798WT). Cap and mix. Wait 5 minutes.
4. Insert test tube into Octa-Slide Viewer (1100) with
Ammonia Nitrogen Octa-Slide Bar (3438) inserted.
5. Record as ppm Ammonia Nitrogen (NH
3
-N).
NITRITE NITROGEN
1. Fill test tube (0106) to 2.5 mL line with sample water.
2. Dilute to 5 mL line with *Mixed Acid Reagent
(V-6278).
3. Use 0.1g spoon (0699) to add 0.1g of *Color
Developing Reagent (V-6281). Cap and mix for 1
minute. Wait 5 minutes.
4. Insert test tube into Octa-Slide Viewer (1100) with
Nitrite Nitrogen Octa-Slide Bar (3437) inserted.
5. Record as ppm Nitrate Nitrogen (NO
2
- N).
5. Record as ppm Nitrite Nitrogen (NO
2
-N). ALKALINITY
1. Fill titration tube (0608) to 5 mL line with sample
water.
2. Add 4 drops of *BCG-MR Indicator (2311-EG). Cap
and mix. Sample will turn blue-green.
3. Fill Direct Reading Titrator (0382) with *Alkalinity
Titration Reagent B (4493DR).
4. Ti trate sam ple un til blue-green color changes to pink.
5. Re cord as ppm Alkalinity (CaCO
3
).
5. Record as ppm Alkalinity (CaCO
3
). CARBON DIOXIDE
1. Fill titration tube (0608) to 20 mL line with sample
water.
2. Add 2 drops *Phenolphthalein Indicator, 1% (2246). If
sample turns red, no free carbon dioxide is present. If
colorless, proceed to Step 3.
3. Fill Direct Reading Titrator (0380) with *Carbon
Dioxide Reagent B (4253DR).
4. Titrate sample until faint pink color persists for 30
seconds.
5. Record as ppm Carbon Dioxide (CO
2
).
CHLORIDE
1. Fill titration tube (0608) to 15 mL line with sample
water.
2. Add 1 drop *Phenolphthalein Indicator, 1% (2246). If
sample is colorless, proceed to Step 3. If sample turns
pink, add *Sulfuric Acid, 0.5N (6090) one drop at a
time until pink color disappears.
3. Add 3 drops *Chloride Reagent #1 (4504). Cap and
mix. Sample will turn yellow.
4. Fill Direct Reading Titrator (0382) with *Chloride
Reagent #2 (4505DR).
5. Titrate sample until yellow color first changes to orange
or orange-red.
6. Record as ppm Chloride (Cl).
HARDNESS
1. Fill titration tube (0608) to 12.9 mL line with sample
water.
2. Add 5 drops of *Hardness Reagent #5 (4483). Cap and
mix.
3. Add 5 drops of *Hardness Reagent #6 (4485). Cap and
mix. Sample will turn red.
4. Fill Direct Reading Titrator (0382) with Hardness
Reagent #7 (4487DR).
5. Titrate sample until red color changes to clear blue.
6. Record as ppm Total Hardness (CaCO
3
).
LaMOTTE COM PANY
Helping Peo ple Solve An a lyt i cal Challenges SM PO Box 329 · Chestertown · Mary land · 21620 · USA
800-344-3100 · 410-778-3100 (Out side U.S.A.) · Fax 410-778-6394 · www.lamotte.com
63634-03-SF · 2.07