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3 installation, 1 installing the variocell, Installing the variocell – Metrohm Variocell for Bioscan – 6.5331.1X0 User Manual

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3 Installation

IC Equipment set Variocell for Bioscan

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3 Installation

3.1

Installing the Variocell

Caution

Never switch the Variocell on, when...

… it is not completely connected, or

… if it is not simultaneously being rinsed by a conductive eluent.

The working electrode or other electronic components may be dam-
aged.

Installing the Variocell

1 Removing the reference electrode

Unscrew the reference electrode.

Removing the reference electrode prior to the filling of the cell pre-
vents air bubble formation while the reservoir is being filled.

2 Connecting the eluent inlet

Use a pressure screw 6.2744.014 to attach the column connection
capillary (attached to the separation column) to the eluent inlet (1-6)
of the Variocell.

In order to avoid dead volume while doing so, slide the capillary up
to the stop in the cell and then rotate the pressure screw in until the
capillary is fixed in place. Do not however tighten the pressure screw
too much, as this could cause damage to the thread.

3 Connecting the eluent outlet

Use a pressure screw 6.2744.014 to attach one end of the sup-
plied PTFE capillary 6.1803.030 to the eluent outlet (1-7) of the
Variocell.
In order to avoid dead volume while doing so, slide the capillary
up to the stop in the cell and then rotate the pressure screw in
until the capillary is fixed in place. Do not however tighten the
pressure screw too much, as this could cause damage to the
thread.