Labnet Deluxe ProBlot Hybridization Systems (6, 12, 12S and 24) - Manual (Page 5)

-Temperature Controller and Temperature Set – The controller has a 3-digit display for
displaying chamber temperature or set point information. UP and Down arrow pads are
used to change the set point and controller mode of operation. To enter the set point mode
of operation on the controller, press either the UP or DOWN arrow pad one time. The
display will start to blink, going from bright to dim. While blinking, the display is showing
the set point. To change the set point, use the UP and DOWN arrow pads. If the arrow
pads are not pressed for five (5) seconds, the display will stop blinking and will read the
chamber temperature. Allow at least one hour for the chamber temperature to stabilize and
24 hours for optimum stabilization.
-Calibration- The oven is calibrated at 65C at the factory. The oven can be re-calibrated
after the chamber temperature has stabilized at the set point for several hours. Suspend a
certified reference thermometer in a hybridization bottle such that it does not touch the
glass bottle sides or (less accurate) tape the reference thermometer to the outside wall of a
hybridization bottle. Mount the bottle on the rotisserie (and a second bottle for balance)
and run the unit at the desired temperature for 2 hours. Compare the oven display to the
reference thermometer. If there is an unacceptable difference, put the controller into cali-
bration mode by pressing both the UP and DOWN arrow pads at the same time until the
two outside decimal points begin to flash. While the decimal points are flashing, the display
can be calibrated to match the reference thermometer by pressing the UP or DOWN arrow
pads until the display reads the correct value. Allow the incubator to stabilize again, and
recalibrate if necessary.
-Heating Indicator-This indicator will illuminate green when the controller is calling for heat
from the heater. This indicator will be on continuously while the oven heats up to the set
temperature and will then cycle on and off at the set temperature.
-Rotisserie Power Switch – This switch turns on the power to the rotisserie and is used to
start and stop the rotisserie.
-Rotisserie Speed - This knob controls the rotisserie speed. Clockwise rotation increases
speed. The oven has a nominal speed range of 4 to 20 rpm.
-Shaker Timer (Problot 12S only) – The timer controls the power to the shaker mecha-
nism.
Turning the knob counter clockwise to the RUN position sets the shaker for continuous
operation. Turning the knob clockwise sets the shaker for timed operation in a timed cycle
from 0 to 120 minutes. The knob can be rotated counter clockwise to the 0 or RUN position
any time.
-Shaker Speed (Problot 12S only) – This knob controls the shaker speed. Turning the
knob
clockwise increases the speed from 10 to 300 rpm.
OPERATING FEATURES
-Rotisserie – The rotisserie is stainless steel and has clips to hold hybridization bottles.
The standard rotisserie will hold either six or twelve large (300mm) bottles and double the
number of small (150mm) bottles. The clips may be squeezed inward to also hold 50ml
tubes. Optional rotisseries that hold other styles of bottles or vertical 50ml tubes are avail-
able. Always load the rotisserie with an even number of bottles in a balanced pat-
tern.