C.B.S. Scientific C-DASG-400-50 - Manual (Page 9)

9 Mega-Gel Instructions 12/11
7. Apply gel solution to gel plate sandwich using a
syringe or pipette. If using a stacking gel, pour
desired height of running gel, then overlay a small
amount of dH
2
O or 0.1% SDS solution to top of
gel. After polymerization, rinse with buffer, add
stacking gel solution and insert comb. For regular,
single percentage gels, add polyacrylamide solution
to correct height, and insert comb. Allow gel to
polymerize, usually 20-30 minutes. Extra gel
solution in pipette or syringe can be monitored to
test polymerization of gel mix.
Leave the comb in the plate sandwich until just
before sample loading
8. Disassembly. Hold the clamped plate assembly with
one hand. Remove the gasket by starting at one of the
top ends and pulling “up and out” on the gasket until
it releases from the plate, up to the bottom of each
of the white clamps. When each clamp is reached
DO NOT remove it, instead feed the gasket down
through the clamp body and repeat pulling up and out.
Continue feeding until the gasket is fully detached.
9. Bottom spacer must be removed before electrophoresis. Using a
spatula, needle or extra spacer gently push down on angled part of bottom
spacer and remove. If gel is not to be used immediately, wrap entire plate
sandwich with plastic wrap tightly to seal and store at 4°C for up to a week.