C.B.S. Scientific C-DASG-400-50 - Manual (Page 7)

7 Mega-Gel Instructions 12/11
Section 3
Assembly
3.1 Unit Assembly for Mega-Gel
1. Place the Mega-Gel on a level work surface in an authorized work area. Attach two guide rods to base. Tilt
base on its side, place stainless steel screw through countersunk hole and screw the guide rods onto the base
2. Secure lower reservoirs to base by tightening the four black knurled thumb screws.
3. Once guide rods are secure and apparatus is flat on laboratory bench, slide the divided upper reservoir over
the two guide rods. To adjust upper reservoir to desired height, align notch in glass plate with upper reservoir
fascia. Tighten the two rosette knobs on the upper reservoir until secure. Level apparatus using leveling feet
in base.
4. Upper and lower reservoirs are shipped with drain plugs installed. Verify that the drain plugs are
secure. If you plan to use accessory Quick-Disconnect fittings (Cat.# QDC-B and QDC-I), remove the
drain plugs. Wrap the end of the Quick Disconnect Insert with Teflon tape and thread into the drain
hole. Be sure the Quick Disconnect Body is secure and the Quick Disconnect Insert snaps into place.
5. Dual units include a rotating base. Align cut-out in bottom of base with rotating base. Use stationary pin to keep
unit form rotating.
6. Level the assembled sequencer using the four leveling feet supplied. Each sequencer is shipped with an
attached bubble level.
3.2 Glass Plate Preparation
1. Select a pair of glass plates (if using a notched set, one notched and one unnotched back plate). Be sure
the edges of the plates are nick or chip free. Using a diamond pencil, mark one side of the notched
plate. Use this marker to remember the side of the notched plate that will always be clamped to the
upper reservoir gasket. This is done because the side of the glass that is clamped to the gasket has
a “silicone smudge” that has a adverse effect on the pouring of acrylamide gels even after washing.
2. It is vital that these plates are absolutely CLEAN ! Wearing gloves (and rinsing off any residual powder), clean
the glass plates thoroughly with a non abrasive detergent and a plastic scouring pad. The cleaning solution
should not leave a soap residue when rinsed thoroughly.
3. Rinse the glass plates thoroughly with de ionized water and wipe dry.
4. Immediately before assembling the glass plate ‘sandwich’, rinse the inside surfaces with 95% ethanol and wipe
dry with a lint-free paper towel. Avoid touching the inside surfaces with your fingers. The wearing of gloves
is highly recommended. Impeccably clean, dust free plates are essential for pouring gels without bubbles.
5. Assemble the glass plate sandwich using gel wrap gasket as described in section 3.3.
• 2 ea 100 well combs
• 2 sets Gel Wrap™ spacers
• 2 sets Gel Wrap™ glass plates
• 2 wedge plate separators
• 2 temperature strip, Cat.# EGT-100
• 2 Gel Wrap™ Gaskets
• Rosette knobs (2)