C.B.S. Scientific C-DASG-400-50 - Manual (Page 5)

5 Mega-Gel Instructions 12/11
SECTION 1
General Information
1.1 Introduction
This Dual Adjustable Mega-Gel is designed for high-throughput microsatellite mapping (1) with a capacity of up to 200 samples
per run. The glass plate sets include a low fluorescence back plate, making it ideal for fluorescence-based assays. This feature
allows the bands to be visualized without the time-consuming process of removing the gel from the glass plate sandwich
and staining. Alternatively, ethidium bromide is added to the lower reservoir prior to electrophoresis and the low fluorescence
glass allows direct UV visualization and photography of the results without gel removal. To maximize throughput, multiple
amplification products can be loaded simultaneously or successively in the same well (see ref. 1, Wang Paper, pg. 1830).
To further enhance screening productivity, the system includes two 100 well combs that feature microtitre spacing for multi-
channel pipettor loading. This adjustable System offers glass plate sizes of 22cm and 32cm(h).
1.2 Specifications
Constructions:
Upper buffer chamber Acrylic
Lower buffer chamber/base Acrylic
Safety covers Acrylic
Electrodes Pure platinum wire .010” diameter
DC Power leads Silicone 7500VDC, 200mA, 65ºC 200mA, 65ºC
Glass plates Soda-lime float glass or Low fluorescent borosilicate glass
Combs Polycarbonate or Mylar
Spacers Polycarbonate, Mylar or PVC
White spring clamps Polypropylene, stainless steel
Table 1: Features of Dual Mega-Gel System C-DASG-400-50
Model # Single or Dual Plate Dimensions
C-DASG-400-50 Dual 50cm(w) x 22cm(l)
50cm(w) x 32cm(l)
Table 2: Specifications
Model # C-DASG-400-50
Shipping Weight 2 boxes: 38 lbs and 15 lbs
Overall Size 34cm(d) x 60cm(w) x 44cm(h)
Recommended buffer volume
Cathode reservoir
Anode reservoir
2 liters total
500mls each side
500mls each side
Distance between electrodes 36cm
Recommended Running Conditions 350 VDC / 105mA / gel / 1.5mm thickness
1.3 Safety
Power to the Mega-Gel unit is to be supplied by an external DC voltage power supply that must be ground isolated
so that the DC voltage output floats with respect to ground.
Current to the unit, provided from the external power supply, must enter the unit through the lid assembly, providing
a safety interlock to the user. Current to the unit is broken when the lid is removed. Do not attempt to use the unit
without the safety lid, and always turn the power supply off before removing the lid, or when working with
the unit in any way. Follow safety precautions specified by the power supply manufacturer.