C.B.S. Scientific C-DASG-400-50 User Manual
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Mega-Gel Instructions 12/11
4.5 References and Suggested Reference Literature
1. Wang, Shi, Carlson, Cregan, Ward, Diers.
A Low-Cost, High-Throughput Polyacrylamide Gel
Electrophoresis System for Genotyping with Microsatellite DNA Markers. Crop Science (43:1828-1832)
2.
MOLECULAR CLONING A Laboratory Manual, 2nd edition, ed. by J. Sambrook, E.F. Fritsch and T. Maniatis,
Cold Spring Harbor Laboratory Press, 1989.
3.
CURRENT PROTOCOLS IN MOLECULAR BIOLOGY ed. by F.M. Ausubel, R. Brent, R.E. Kingston, D.D. Moore,
J.G. Seidman, J.A. Smith and K. Struhl, Greene Publishing Associates and Wiley-Interscience, New York, 1989.
Section Section 5 Maintenance of Equipment
5.1 Care and Handling
The components of the Mega-Gel are fabricated from acrylic, Delrin®, Polycarbonate, glass and anodized aluminum.
As with any laboratory instrument, adequate care ensures consistent and reliable performance.
After each use, rinse buffer reservoirs, combs, spacers, clamps and glass plates with deionized water. Wipe dry
with a soft cloth or paper towel, or allow to air dry. Whenever necessary, all components may be washed gently with
water and a non abrasive detergent, and rinsed and dried as above. Never use abrasive cleaners, window sprays
or scouring pads to clean the components, as these cause damage to the plastic surfaces.
• Do not autoclave or dry-heat sterilize the apparatus or components.
• Do not expose the apparatus or components to phenol, acetone, benzene, halogenated hydrocarbon
solvents or alcohols.
• Avoid prolonged exposure of the apparatus or components to UV light.
5.2 Maintenance
The following simple, routine inspection and maintenance procedures will help ensure both the safety and the
performance of the Mega-Gel. For ordering information of replacement parts call 1-858-755-4959.
• Inspect electrical connections and power cords regularly. If the banana plug connectors to either the unit or the
power supply do not exhibit reasonable friction or can be rocked easily, replace the plugs or power cords.
• If power cords show any signs of wear or damage (e.g. cracks, nicks, abrasions, or melted insulation), replace
them immediately.
• Examine the banana jacks to ensure that they are tight and free of corrosion. If banana jacks are corroded,
they should be replaced.