C.B.S. Scientific PP-048-202-SS - Manual (Page 5)

Optimizer PCR Workstation Instructions 3/2013 www.cbsscientific.com 4
General Information
1.1 Introduction
The PCR Workstation ® is designed to provide an optimal environment for performing PCR amplification reactions.
The Workstation interior can be irradiated prior to use, blocking replication of potentially contaminating DNA
sequences (1,2). The protected area within the Workstation limits exposure of the experimental set-up to the
open lab environment, decreasing the chances of cross or airborne contamination.
The PCR Workstation ® is available in six different models. The 3 sizes available are 2 feet deep x 2 feet high,
with widths of 30, 36 or 48 inches. Each size can be ordered with either a single or dual UV light built into the
ceiling. For added convenience, a twelve-hour timer controls the UV irradiation dosage, and can be set to a
pre-determined time for decontamination. The PCR Workstation can be placed on a lab bench, or turned into
a moveable work area by ordering an accessory cart with locking casters.
1.2 Intensity of UV Light (254nm) for Single and Dual UV Bulb PCR Workstation ®
Some labs may require the intensity of the Dual UV Bulb PCR Workstation ® to adequately decontaminate their
hood. The Dual Bulbs deliver twice the intensity of UV light than that of the single, and will help irradiate areas
that might otherwise be inaccessible. The Dual UV Bulb format is recommended when the researcher desires
to use the Workstation to decontaminate apparatus and reagents. The light from one bulb may not adequately
access shadowed areas created by these items and the intensity of reflected UV light is greatly weakened. To
help solve this problem, the two UV bulbs are mounted apart from each other on the ceiling, maximizing the
contents of the Workstation that will receive direct UV irradiation as opposed to reflected. The Dual UV Bulb
PCR Workstation ® may also be required to deliver the sufficient UV dosage needed to prevent replication of
certain types of contaminating DNA. For example, inactivation of dry DNA requires more UV exposure than that
of DNA in solution. To determine the best suitable UV irradiation dosage for the type of DNA being used please
refer to table 3.2. on page 10, combined with the values given in Fairfax et al (3).