C.B.S. Scientific PP-048-202-SS - Manual (Page 14)

Optimizer PCR Workstation Instructions 3/2013 www.cbsscientific.com 13
Table 3.2 Intensity of UV light for Single and Dual UV Bulb PCR Workstation ®
Using these values and the information supplied in Ref. 3 (Section 3.3), it is possible to calculate desired
exposure times considering the type and source of contaminating DNA. To confirm UV dosage refer to Section
4.2.
P-030-02
Single
UV Bulb
Workstation
P-036-02
Single
UV Bulb
Workstation
P-048-02
Single
UV Bulb
Workstation
P-030-202
Dual
UV Bulb
Workstation
P-036-
202 Dual
UV Bulb
Workstation
P-048-
202 Dual
UV Bulb
Workstation
µW/cm 2 200 200 400 400 400 800
3.3 References
1. Sakar,G., and Sommer, S, (1990) Nature 343 p. 27.
2. Ou,C-Y, Moore, J.L. and Schochetman, G Biotechniques (1991) 10:4 p. 442-445.
3. Fairfax, M.R., Metcalf, M.A., Cone, R.W., (1991). PCR Methods and Applications. Cold Spring Harbor
Laboratory Press, Cold Spring Harbor, New York. 1:142-143.
4. Lehninger, A.L., Nelson D.L., and Cox, M.M., (1993) Principals of Biochemistry. Worth Publishers, New
York, NY pages, 342, 816, 832, 837
SECTION 4
Maintenance of Equipment
4.1 Care and Handling
The plastic components of the PCR Workstation ® are fabricated from acrylic. As with any laboratory
instrument, adequate care ensures consistent and reliable performance. After each use, wipe black formica
or stainless steel base with 50% ethanol. Wipe down interior space with 25% ethanol. Wipe dry with a soft
cloth or paper towel, or allow to air dry. Whenever necessary, all components may be washed gently with
water and a non-abrasive detergent, and rinsed and dried as above. Never use abrasive cleaners, glass
cleaning sprays or scouring pads to clean the components, as these will damage the unit and components.
UV exposure may over time result in slight discoloration of the workstation and equipment decontaminated
in the workstation. This is a cosmetic change, and will not affect the functionality of the equipment.
Additional precautions:
• Do not autoclave or dry-heat sterilize the apparatus or components.
• Do not expose the apparatus or components to phenol, acetone, benzene, halogenated hydrocarbon
solvents or undiluted alcohol's.
• Do NOT treat with diethylpyrocarbonate (DEPC)-treated water for extended periods at 37°C. A
brief rinse with DEPC-water is sufficient after a thorough wash, followed by a quick rinse in 70%
ethanol.
4.2 Measuring Strength of UV Bulbs using a UV Meter
1. The life span of a new UV bulb is approximately 1,000 hours. However, bulb life varies
depending on several factors such as number of starts, duration of illumination and
original condition. The best way to determine UV dosage at any place in the workstation
is to measurer UV output with a simple metering device.