Eppendorf Repeater plus - Manual (Page 9)

9
Multipette® plus · Repeater® plus — Operating manual
4.3.2 Discard first dispensing step (reverse stroke).
1. Wipe the Combitip tip using lint-free cell tissue.
2. Place the Combitip tip on the tube wall.
3. Actuate the dispensing lever once and discard the liquid (reverse
stroke).
This step, which brings the system to the basic position, should be
completed independent of the volume setting.
The display is no longer blinking, and displays the dispensing
volume. The Multipette plus/Repeater is ready for operation, the
full range of dispensing steps are available.
Comments
•
If you do not fully fill the Combitip, the display will not blink. Press
the dispensing lever fully downward as often as necessary until
liquid escapes from the Combitip plus. Discard the liquid. Then,
you can dispense the set volume.
•
Between dispensing steps, you can change the volume using the
volume selection dial.
•
Pressing the filling lever too quickly may create a too high vacuum.
The liquid is enriched with air bubbles which could cause
dispensing inaccuracies.
With a volume selection dial setting larger 5, the aspiration of a
large air bubble can lead to drip formation after each dispensing
step, particularly with 10, 25 and 50 mL Combitips.
•
A small air bubble in the upper cylinder chamber has no effect on
dispensing.
•
The Combilong/Combitube is an aspiration aid for the Multipette
plus/Repeater plus. The Combilong/Combitube enables direct
liquid aspiration from bottles.
4.4 Dispense
1. Place the tip of the Combitip against the wall of the tube.
2. Push the dispensing lever up to the stop.
After the Combitip has been refilled, discard the first dispensing step.
4.5 Release Combitip
1. Hold the Combitip in a tube and push the filling lever right to the
bottom.
The residual liquid is emptied.
2. Hold the Multipette plus/the Repeater over a waste container.
3. Press both ejection keys at the same time.
The Combitip (in the case of 25 mL and 50 mL including adapter)
is released from the Multipette plus/the Repeater plus. The display
goes out.
4. On the Combitip 25 mL and 50 mL: unscrew the reusable adapter
from the Combitips.
The adapter is a wear part. If significant wear is apparent, do not
reuse the adapter.
Hint!
If the remaining liquid is not enough for complete dispens-
ing of the set volume for the last dispensing step, the resid-
ual stroke lock automatically prevents an insufficient
amount from being dispensed.