Eppendorf Mastercycler pro - Manual (Page 43)

Programming
Mastercycler pro
English (EN)
43
TSP/ESP heated
lid Preheat heated lid
• Mastercycler pro: Switch TSP on and off.
When TSP is activated, the heated lid is heated to the set temperature, while the
block is maintained constant at 20 °C. The program is started after the lid
temperature has been reached.
• Mastercycler ep: Switch ESP on and off.
When ESP is activated, the heated lid is first heated to the set temperature before it
is lowered onto the tube surfaces. The program is started after the heated lid has
been lowered.
Switch off lid at
low block
temperature Lid heater with automatic switch-off
• Activated (default setting): The lid heater switches off if program steps have block
temperatures of < 15 °C.
• Deactivated: The lid heater switches off after the end of the program. If the last
command in a program is a hold step, the heated lid remains switched on until the
Enter key is pressed.
Note: This option is useful if you have inserted hold step as the last program step in
order to automatically cool the samples after the PCR.
Temp. mode Block temperature control mode
• block (default setting): The temperature of the thermoblock is directly controlled by
the current temperatures measured at the integrated sensors.
Use this setting for Fast PCR, for low reaction volumes (< 20 μL) and for templates
with a low G/C content. With this setting you achieve the shortest program
runtimes.
• safe : Block temperature control mode for difficult templates and large reaction
volumes.
Use this setting for sample volumes of 50 μL or more or for amplifying samples
with a high G/C content. The program runtimes are a bit longer than with the
fast
setting.
• fast : Block temperature control mode for standard applications.
Use this setting for sample volumes between 20 μL and 50 μL or if the block setting
only results in a weak amplification. The program runtimes are a bit longer than
with the
block setting.
Simulate
Mastercycler
gradient With this setting it is possible to simulate the temperature control behavior of the
Mastercycler 5333 or Mastercycler gradient 5331. This allows you to transfer
programs from these cycler models without making adjustments to the
Mastercycler pro(see Transferring programs from the Mastercycler 533x on p. 64).
If this option is deactivated, Mastercycler 5333/5331 programs will have to be
adjusted for the Mastercycler pro due to the different temperature control behavior.
This may influence the PCR results.
Impulse This is only displayed if under Mastercycler the pro S or ep gradient S setting were
selected.
If this option is activated, a higher heating rate (pulse) is achieved during the first
temperature control step. This can reduce non-specific PCR product formation.