Eppendorf Eporator - Manual (Page 16)

Operation
Eppendorf Eporator®
English (EN)
16
5.2.4 Temperature
• Cooling electroporation cuvettes: Electroporation of microorganisms produces the
best results at low temperatures (0-4 °C). Cool the electroporation cuvettes to 0 °C
before electroporation. Remove residual moisture from the electroporation cuvette
before inserting it in the Eporator.
5.3 Performing electroporation
5.3.1 Switching on the device

Press the mains power switch on the rear of the device to switch on the device.
5.3.2 Inserting the cuvette
The integrated cuvette holder is located at the front of the device to the right of the control
panel.
Proceed as follows:
1. Remove the cuvette holder from the device.
The cuvette holder is now freely accessible for the insertion of the electroporation
cuvette.
2. Remove the electroporation cuvette from the individual packaging.
3. Remove the lid of the electroporation cuvette.
4. Fill the electroporation cuvette with the sample. The slit between the plate electrodes
must be filled without bubbles.
5. Place the locking lid on the electroporation cuvette.
6. Insert the electroporation cuvette into the cuvette holder with the plastic nose pointing
towards the back.(Fig. 5-3 on p. 17)
To increase the efficiency of the electroporation, the electroporation cuvette can
be cooled prior to filling. Remove residual liquids from the cuvette before further
use.
NOTICE! Damage to device due to penetration of liquids.
Liquid can enter the device during electroporation with cuvettes without lids.

Only cuvettes with square lids may be used for electroporation.