Eppendorf Concentrator plus - Manual (Page 24)

5 Operation
26
Concentrator plus / Vacufuge® plus — Operating manual
5.3.5 Loading swing-bucket rotor
Requirement
• A combination of rotor, bucket and adapter, approved by Eppendorf.
• Two inserted buckets.
• Matching and tested tubes and plates.
• Adapters and plates with a total height of ≤ 27 mm.
1. Check the bucket grooves for cleanliness and grease lightly with pivot grease (order no. Int.:
5810 350.050 / North America: 022634330).
Dirty grooves and pivots prevent buckets from swinging out evenly.
2. Hang the buckets into the rotor.
3. Check that both buckets are hanging properly and can swing freely.
4. When using a plate type for the first time, carry out a manual loading and settling test.
5. Check maximum load (adapter, plate and content) per bucket.
The relevant details can be found on the rotor and in this operating manual (see Rotors on
page 12).
6. Load the bucket symmetrically when inserting adapters and plates.
The plate arrangement shown on the right-hand side is incorrect, as the bucket will not swing
properly.
The plates have slight play in the buckets.
7. Attach and tighten rotor lid.
5.3.6 Close lid of the device
1. Check that the rotor is attached correctly.
2. Close lid of the device.
CAUTION!
Caution! Filling the plates too high can cause overflowing.
During centrifugation the meniscuses in the tubes along the edges of the plates are at an angle.
This is due to the centrifugal forces and cannot be avoided.
Fill the wells of the plates to a maximum of 2/3 of the max. capacity.
WARNING!
Warning! Risk of injury from unsymmetric loading of rotors.
Load rotors symmetrically with identical tubes and/or buckets and plates.
Only load adapters with suitable tubes and/or plates.
Always use tubes and/or plates of the same type (weight, material/density and volume).
Check for symmetric loading by balancing the adapters and tubes and/or plates used with
scales.
WARNING!
Warning! Risk of injury from fingers being crushed.
When closing the device lid, do not reach between the lid and the device or into the latching
mechanism of the lid, as your fingers may be injured.
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