Multichannel Systems MEA1060-Inv-BC Manual - Manual (Page 30)

MEA Amplifier with Blanking Circuit for Inverse Microscopes
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5.3 Mounting the MEA Probe and Grounding the Bath
Warning: Do not use too much force. Otherwise you could damage the delicate MEA
or contact pins of the amplifier. Put the lid of the amplifier only onto a dry and clean
area with the bottom side down. Otherwise, you can easily damage the contact pins.
Important: Click Change MEA in the MEA_Select program before you remove the shielding or
open the amplifier. Deactivate the Change MEA mode only after having completely set up the
amplifier, including grounding the bath and shielding the amplifier. Otherwise, it can take very
long (several seconds to minutes) until the amplifier has recovered and is ready for operation.
Mounting the MEA Probe
1. In the MEA_Select program, click Change MEA.
All electrode inputs are connected with the amplifier's ground. This helps avoiding
a large DC offset and reduces the baseline restoring time significantly. See also the
chapters "Operating the MEA Amplifier", and "Signal Amplification and Filters".
2. Place the MEA amplifier as shown on the photo. Open the lid of the MEA amplifier.
3. Place the MEA probe inside.
Important: MEA chips are not symmetrical! That is, why the writing (NMI) on the MEA chip should
be on the right side. Otherwise, the MEA layout will not match with the pin layout of the channel
map in MC_Rack. This is very important for MEAs with internal reference electrode, for example.
4. Replace the lid and close it carefully. Do not unpress the Change MEA button until
you have grounded the bath and replaced the shielding, that is, until the setup has
been completely finished.
Warning: Spilled liquid can damage or even completely destroy the electronics of the
MEA amplifier. Please be careful when setting up your perfusion system and when starting
the perfusion. Take care that the flow rates of the inlet and outlet flow match so that
flooding of the amplifier is efficiently prevented.