Ocean Optics CUV-cce - Manual

CUV-CCE Electrophoresis Sample Cell
The CUV-CCE E LECTROPHORESIS S AMPLE C ELL for chromatography and capillary electrophoresis is an
optical fixture for measuring the UV absorbance of fluids in chromatography or capillary electrophoresis
systems. The cell is attached on-line, i.e., the light is projected through the sides of fused silica tubing without
violating the tube integrity. For this reason, there are no pressure limitations associated with the device. The
cell can accommodate fused silica tubing up to 500
µ
m in diameter. The user must provide a clear optical
window. For standard polyimide-jacketed tubing, this can be accomplished by burning off a short section of the
jacketing. The CUV-CCE comes with F230 0.016" ID tubing sleeves. If your tubing is a different size, you can
order sleeves with different inner diameters.
The cell fixture is made from a standard 10-32 PEEK TM Cross (Upchurch # P-729) with a 0.02" through-hole,
10-32 coned female threads and (4) F-300 PEEK finger-tight fittings. The optical fibers are aluminum-jacketed,
300-
µ m diameter, solarization-resistant, silica-core/silica-clad UV waveguides. The optical fibers are inserted
facing each other across the sample tubing, and secured with the same F230 0.016" ID tubing sleeves and
finger-tight fittings.
Operation
Eliminating Polyimide Jacketing
1. Prepare the silica sample tube by burning off the polyimide jacketing with a match or butane lighter.
2. Make sure the tube has completely cooled and then rinse the tubing to remove any burn residue, particles, etc.
Inserting the Sample Tubing
1. Insert the sample tubing through a finger-tight 10-32 fitting and tubing sleeve. Carefully feed the tube
through the through-hole of the cross, until the clear window is approximately at the center point of
the cross.
2. Tighten the 10-32 fitting until the tube is just snug enough to stay in place. Do not over-tighten.
3. Install the other 10-32 fitting and sleeve on the tubing
and into the cross. Leave this fitting loose for now.
Configuring the Optical Fibers
1. The optical fiber may already be installed in the
cross. If it is not, insert the fiber through a 10-32
fitting and tubing sleeve. Insert the fiber into the
through-hole of the cross, pushing it gently until it
makes contact with the sampling tube. Back the
fiber off just enough to leave the sample tube free
to manipulate. Tighten the finger-tight fitting to
hold the fiber firmly. Do the same for the other
fiber as well.
2. Connect one fiber to the SMA connector on a
deuterium source (we recommend the DT-1000
for UV/VIS work, or the D-1000 for UV only).
3. Connect the other fiber to the spectrometer (we
recommend a unit with an L2 lens and either
grating #1 or #2, a 25
µ
m slit, the UV2 detector
upgrade and an OFLV 200-850 order-sorting filter).
Operating Instructions: CUV-CCE
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Sample tube
!
"
Al fiber