Ocean Optics KR-1 Krypton - Manual (Page 3)

KR-1-CAL Installation and Operation Instructions
008-00000-KR1-01-1209 3
Note
If the spectrometer does not have a slit and your experiment requires you to use fibers
of varying diameters, you will need to perform a wavelength calibration after
changing fibers. You should perform a wavelength calibration each time you unscrew
the fiber from the spectrometer and change fiber size.
3. Move the On/Off switch on the KR-1 (next to the SMA 905 Connector) to the On position.
The red LED will illuminate to indicate that the KR-1 is powered on.
You have now configured the KR-1 for use.
Calibrating With the Light Source
The information in this section explains how to calibrate your spectrometer’s wavelength using the
KR-1 light source.
About the Wavelength Calibration
You are going to be solving the following equation, which shows that the relationship between pixel
number and wavelength is a third-order polynomial.

p
= I + C
1
p + C
2
p 2 + C
3
p 3
Where  = the wavelength of pixel p,
I = the wavelength of pixel 0,
C
1
= the first coefficient (nm/pixel),
C
2
= the second coefficient (nm/pixel 2 )
C
3
= the third coefficient (nm/pixel 3 )
You will be calculating the value for I and the three Cs.
Calibration Requirements for Ocean Optics
Spectrometers
To re-calibrate the wavelength of your Ocean Optics spectrometer using the KR-1, you will need the
following items:
 KR-1 Krypton Calibration Light Source
 Ocean Optics spectrometer and its manual
 An optical fiber (for spectrometers without a built-in slit, a 50-
 m fiber works best)
 A spreadsheet program (Excel or Quattro Pro, for example) or a calculator that performs third-
order linear regressions