Ocean Optics Apex Install - Manual (Page 17)

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Appendix A
Calibrating the Wavelength of
Apex
Overview
This appendix describes how to calibrate the wavelength of your spectrometer. Though each spectrometer
is calibrated before it leaves Ocean Optics, the wavelength for all spectrometers will drift slightly as a
function of time and environmental conditions. Ocean Optics recommends periodically recalibrating the
Apex.
About Wavelength Calibration
You are going to be solving the following equation, which shows that the relationship between pixel
number and wavelength is a third-order polynomial:
λ
p
= I + C
1
p + C
2
p
2
+ C
3
p
3
Where:
λ
= the wavelength of pixel p
I = the wavelength of pixel 0
C
1
= the first coefficient (nm/pixel)
C
2
= the second coefficient (nm/pixel
2
)
C
3
= the third coefficient (nm/pixel
3
)
You will be calculating the value for I and the three Cs.