Hoefer SUB Series - Manual (Page 30)

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p22
Running the Gel
1 Samples should be mixed with a suitable marker
dye or loading buffer prior to loading. This
aids sinking of the sample into the well, and
visualization of how far the samples have run.
Consult your laboratory manual for details on
loading buffers.
2 Load the samples into the wells taking care not
to damage the sides or bottoms of the wells.
Replace the lid correctly BEFORE connecting the
leads to the power supply.
3 Set the voltage and current to suit the
electrophoretic application. As a guide, to obtain
the optimum resolution of DNA fragments,
agarose gels should not be run greater than
5V/cm.
4 Long runs may require buffer re-circulation, to
prevent over heating and or buffer depletion.
Re-circulation ports are provided in the SUB13,
SUB15, SUB20, SUB20C, SUB25, SUB25C
and SUBHT units.
5 The Cooled gel units have a built-in cooling
block in the base. The side connectors can be
attached, via tubing, to the mains water supply
or to a circulating water bath.
IMPORTANT! Do not exceed the
recommended voltage or current
as this may result in poor band
resolution and may result in
damage to the unit.
IMPORTANT! When re-circulating
buffer, remember that the buffer
flowing through the tubing is live.
Take all necessary precautions.
Warn other workers in the vicinity
of the potential hazard. Seek the
advice of your safety officer.