Hoefer SUB Series - Manual (Page 12)

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p4
Static Temperature Regulation
1 Attach a short length of rubber hose to
each connector.
2 Incline the unit at an angle of approximately
45 degrees with the ports uppermost.
3 Use a funnel to fill the Cooling Platform with
deionized water containing 0.02% (w/v) sodium
azide (preservative to prevent bacterial and
algal growth).
4 When filled, keep the unit inclined and attach
clamps to each piece of rubber hose.
5 The unit can be cooled before an electrophoresis
run if required.
DO NOT FREEZE.
Active Temperature Regulation
1 Attach a short length of rubber hose to
each connector.
2 Attach one end of the rubber hose to the outlet
port of a circulating water bath/flow cooler and
the other end to the inlet port. Alternatively
attach one end of a rubber hose to a water
supply and allow the other rubber hose drain
to waste.
3 The maximum recommended water flow rate is
1 Liter/min. DO NOT exceed this figure.
4 If you are using a circulating water bath/flow
cooler, which exceeds this flow rate, you can
attach a T-connector in line. One branch of
the connector can return water to the bath
and the other can flow to the Cooling Platform
and incorporate a flow regulator such as an
adjustable tubing clamp. Measure and adjust the
flow rate before attaching the line to the gel unit.