Hoefer HE99X - Manual (Page 16)

•
p8
After electrophoresis
1
Important! Always turn off the power supply and
disconnect the leads before removing the lid.
2
If no ethidium bromide was added to the gel or
sample before the run, stain the gel now in a solution
of 0.5 to 1.0 µg/ml ethidium bromide in water
or buffer.
3
Clean the unit as described in the next section.
Care and maintenance
Cleaning
• Never autoclave or heat any component
above 45 °C.
• Never use abrasive cleansers.
• Do not expose the unit to solutions or vapors
of aromatic or halogenated hydrocarbons,
ketones, esters, alcohols (over 30%), or
concentrated acids (over 25%).
The unit is resistant to all common electrophore-
sis buffers, but we recommend a thorough wash-
ing with a mild detergent after each use. Rinse
with distilled water and allow to air dry.
To remove DNase and RNase contamination,
fill the unit with 3% hydrogen peroxide (H
2
O
2
),
soak for 10 minutes, then rinse thoroughly with
DEPC-treated, autoclaved, deionized water.
(Sambrook and Russell, et al. 1:7.82)