Hoefer HE99X - Manual (Page 14)

•
p6
Final casting steps
1
Pour the agarose solution (cooled to 50 °C) onto the
running tray seated in the casting tray. Orient the
comb assembly so that it is at the end of the tray
opposite the direction of migration (typically at the
cathode [–] end, which is marked by a black dot on
the handle). Fit the comb assembly into the slots.
2
Allow a minimum of 30 min for the gel to set, then
remove the comb carefully: partially lift and slightly
tilt the comb at one end and slowly withdraw it from
the gel. (Pulling the comb straight up creates a
vacuum in the wells that may lift the gel out of
the tray.)
3
Lift the running tray out of the casting tray and
transfer it with the gel to the horizontal unit. Orient
the running platform so that the sample will “run to
red”. That is, place the sample wells at the cathode
(–) end, which is indicated by a black dot. A notch on
either side of the running tray centers the tray on the
running platform.