Hoefer SQ33 Sequencer - Manual (Page 17)

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p6
Figure 1. Pouring gels horizontally.
Tap firmly with fingers over
areas such as this
• Hold syringe vertically.
• Move across notched area to maintain a
pool of gel mix.
• Do not overfill.
• Do not attempt to force gel mix down
between plates with the syringe jet.
• When gel boundary has reached the end
remove surplus gel mix from notched
area. Leave just a small amount to
ensure bubbles don’t get trapped under
the comb.
• The comb area may be clamped with
bulldog clips.
• Do not let the clips protrude beyond the
line of the comb otherwise get thick-
ness variation and hence lane distortion
may occur.
• When gel has set carefully remove comb
and insert it teeth first (being careful not
to damage points) so it just contacts the
gel surface.
• The gel is then ready to be attached to
the main unit and loaded with samples.
3 – 5 mm
Notched
Area