Hoefer SG15 - Manual (Page 6)

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p4
8
If it is important that no bubbles disturb the
gradient, watch the delivery carefully and as soon
as the last of the solution has entered the pump
head, stop the pump and remove the tubing from
the receiving container.
9
Flush and rinse all parts thoroughly with distilled
water after use.
Pouring gradients from the top
Filling a container with a gradient (e.g. casting
acrylamide pore gradient gels) can be done either
dense solution first (“from the top”), or light
solution first (“from the bottom”).
To fill from the top:
1
Proceed as described on page 3, putting light (final
or top) solution in the reservoir chamber (step 3) and
dense solution in the mixing chamber (step 5).
2
Place the delivery outlet against the upper edge of
the receiving container. Adjust the pump rate so that
the solution flows evenly down the side in a smooth,
continuous stream. The delivery speed should be slow
enough that the newly arriving solution does not mix
with the underlying solution. Alternatively, using a
rigid cannula at the end of the delivery tubing, hold
the tip of the cannula just above the surface of the
solution, raising it smoothly as the container fills.