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Introduction: hoefer sg15, sg30, sg50, And sg100 gradient makers, The hoefer – Hoefer SG15 User Manual

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1. Introduction: Hoefer SG15,
SG30, SG50, and SG100
gradient makers

The Hoefer

®

SG gradient makers are designed

for producing linear gradients of aqueous solu-
tions ranging in volume from 15–100 ml. The
gradient makers are machined from solid blocks
of acrylic plastic. They are suitable for casting
acrylamide pore gradient gels, casting immobi-
lized pH gradient gels, pouring density gradients
for centrifugal sedimentation separations and
delivering salt gradients for low pressure chro-
matography systems.

The gradient makers have a white Luer outlet
fitting that can take either tubing with female

Fig 1. Hoefer SG gradient maker.

Included but not shown:
Adaptor barbed fitting (1)
22-gauge needle (1)

reservoir (black)
chamber

mixing (front)
chamber

support
rod

stopcock
nut

stopcock
washer

white Luer outlet
connector (4 mm
id tubing)

delivery
stock

connector
channel

connector
stopcock

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