Introduction: hoefer sg15, sg30, sg50, And sg100 gradient makers, The hoefer – Hoefer SG15 User Manual
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1. Introduction: Hoefer SG15,
SG30, SG50, and SG100
gradient makers
The Hoefer
®
SG gradient makers are designed
for producing linear gradients of aqueous solu-
tions ranging in volume from 15–100 ml. The
gradient makers are machined from solid blocks
of acrylic plastic. They are suitable for casting
acrylamide pore gradient gels, casting immobi-
lized pH gradient gels, pouring density gradients
for centrifugal sedimentation separations and
delivering salt gradients for low pressure chro-
matography systems.
The gradient makers have a white Luer outlet
fitting that can take either tubing with female
Fig 1. Hoefer SG gradient maker.
Included but not shown:
Adaptor barbed fitting (1)
22-gauge needle (1)
reservoir (black)
chamber
mixing (front)
chamber
support
rod
stopcock
nut
stopcock
washer
white Luer outlet
connector (4 mm
id tubing)
delivery
stock
connector
channel
connector
stopcock