Hoefer SG15 - Manual (Page 4)

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Luer connectors or tubing of 4 mm inside
diameter (ID). Also included with the gradient
maker is a support rod (that can be screwed into
the body of the gradient maker, allowing it to
be clamped onto a vertical stand), an adapter
barbed fitting (to convert the end of a 2 mm
ID tubing into a female Luer connector) and
a 22-gauge needle (that can be attached to the
white Luer outlet fitting so that it can accept
22-gauge tubing).
2. Generating linear gradients
To generate a linear gradient between two
concentrations, equal volumes of solutions of the
two concentrations are measured into the two
chambers of the gradient maker. As solution is
delivered out of the mixing chamber, an equal
volume flows in from the reservoir chamber
where it is rapidly diluted and mixed to unifor-
mity by a magnetic stir bar. The initial concen-
tration delivered will be that of the solution in
the mixing chamber, the final concentration will
be that of the reservoir chamber. For the most
consistent delivery of gradients, a peristaltic
pump is recommended.
Table 1. Operating ranges for linear gradients
model max total vol min vol/chamber max stir bar length
(ml) (ml) (mm)
SG15 15 3 12
SG30 30 5 15
SG50 50 8 15
SG100 100 16 20