Hoefer SE245 - Manual (Page 9)

•
p7
After polymerization
1
To remove combs (if necessary): cover the top of the
gel with 1X separating or stacking gel buffer, then
work each comb out slowly by gently rocking it side to
side while pulling it out.
2
Remove the gel sandwich from the casting cradle by
loosening the pressure bar screws, tilting the sand-
wich forward, and lifting it out.
3
Rinse the sandwich with distilled water to remove the
buffer and extra gel, then blot the sandwich with a
lint-free tissue.
4
To run gels: Follow the instructions accompanying the
electrophoresis unit.
To store gels: Wrap individual gels in plastic wrap
after adding approx. 5 ml of 1X separating gel buffer
to the top of each sandwich. Alternatively, lay a batch
of gels in 1X separating buffer. Store gels at 4 °C for
up to one week.