Hoefer SE245 - Manual (Page 8)

•
p6
Stacking gel preparation
Pour a stacking gel either while the sandwich is
still in the gel caster or after it is transferred to
the electrophoresis unit (see instrument instruc-
tions). Resolution is optimal when the stacking
gel is poured just before electrophoresis. To pour
a stacking gel in the dual gel caster:
1
Remove the overlay by rinsing the top of the gel
several times with distilled water. Invert the caster to
drain. To ensure a seamless contact between the
separating and stacking gels, remove residual liquid
by blotting one corner with a lint-free tissue.
2
Calculate the volume of stacking gel monomer solu-
tion required. Prepare the stacking gel monomer
solution, deaerate it, and add catalyst and initiator.
3
Pour the stacking gel onto the resolving gel with a
Pasteur pipet.
4
Introduce a comb (at a slight angle) into the sand-
wich, taking care not to trap air under the teeth.
Allow the gel to polymerize.