Hoefer SE245 - Manual (Page 10)

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p8
Troubleshooting
Gel sandwich leaks
• Replace any chipped plates.
• Check plate and spacer alignment and realign
if necessary.
• Check the black gasket for cuts or cracks and
replace if necessary.
• Apply a light film of Gel Seal to the bottom
outside corner of each spacer.
Sample wells damaged or irregular
• Remove air pockets and bubbles before inserting
combs. Slide comb into solution at an angle. If
bubbles persist, remove comb, add more monomer
solution and reinsert the comb.
• Allow a minimum of 1 hour for polymerization of
acrylamide gels.
• Remove the comb at a slight angle and very slowly
to prevent damage to the gel.
Care and maintenance
• Thoroughly wash and rinse all caster components
immediately after use.
• Do not autoclave or heat any part above 45 °C.
• Do not use organic solvents, abrasives, strong
cleaning solutions, or strong acids or bases to
clean any plastic part.
• Do not soak the gaskets. Clean with a mild deter-
gent and allow to air dry.
Clean glass plates and spacers with a dilute solution
of a laboratory detergent such as RBS-35
™ (Pierce
Chemical Co.), then rinse thoroughly with tap and
distilled water. Glass plates can also be treated with
(but not stored in) acid cleaning solutions.
Important! Decontaminate
instrument of all radioactivity
and/or infectious agents
before returning item! Include
documentation to verify that this
has been done.