Labconco Precise HEPA-Filtered Glove Boxes 5220231 - Manual (Page 36)

Chapter 6: Maintaining Your Glove Box
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7. Ensure that the glove box speed control is adjusted to the maximum
setting and the blower is exhausting 100 CFM through the glove box.
8. Position the aerosol generator discharge near the internal opening of
each HEPA filter. Check both filters.
9. Start the aerosol generator. Ensure that the generator is operating
with 1 nozzle on at 10 PSIG.
10. Allow the generator to operate for a minimum of 15 seconds. Place a
1/4" OD sampling tube in the exhaust downstream of the HEPA
filters. The inlet HEPA filter can be checked from the test port on the
exhaust HEPA filter and the exhaust HEPA filter can be checked from
the blower inlet (suction side) test port. Connect this tube to the
“downstream” sampling port of the photometer.
Acceptance
The downstream average penetration shall not exceed 0.005 percent as
measured by the photometer.
Procedure When Using the ATI 2-D or 2-E Photometer
NOTE: Because the downstream side of the HEPA filters cannot be
scanned, a downstream average must be taken.
1. Turn on the photometer and allow it to operate for a minimum 5
minutes. Leave the range switch at “100%” and the valve in the
“CLEAR” setting.
2. Use the screw on the face of the gauge to adjust the meter to 0 if
necessary.
3. If the “INT REF” switch is a square button, depress it. The switch will
remain depressed and illuminated. If the switch is a toggle type
switch, push and hold the switch to the “ON” position. Keep this
switch in the “ON” position through step 4.
4. Turn the “GAIN” knob until a value of 15 is obtained. (Set for 100
CFM, adjust for other airflows.)
5. Turn the range switch to 0.1. Turn the Stray Light knob until the
meter reads “0.”
6. Turn the valve to the “DOWNSTREAM” setting. Place the palm of
your hand over the sampling port of the pistol. There should be a
strong vacuum at this port. Return the valve to the “CLEAR” setting.
7. Ensure that the glove box speed control is adjusted to the maximum
setting and the blower is exhausting 100 CFM through the glove box.
8. Position the aerosol generator discharge near the opening of each
HEPA filter. Check both filters.
9. Start the aerosol generator. Ensure that the generator is operating
with 1 nozzle on at 10 PSIG.
10. Allow the generator to operate for a minimum of 15 seconds. Place a
1/4" OD sampling tube in the exhaust downstream of the HEPA
filters. The inlet HEPA filter can be checked from the test port on the
exhaust HEPA filter and the exhaust HEPA filter can be checked from