ChemoMetec NC-3000 - Manual (Page 13)

12
Identifying the Sub-Population in
the Image Window
One of the advantages of image cytometry is that cells defined in a particular
sub-population can be identified in the image acquired for analysis. This is
done when the image file for the analysis is loaded in main window and sub-
populations have been defined by polygons, quadrants or markers.
Open the desired graph containing the polygon, quadrant or marker
identifying the sub-population.
Select the sub-population by clicking on the gating lines so that it ap-
pears red.
Right-click and choose the appropriate option, for example ‘Add cells in-
side/outside gate to image overlay’.
The events in a scatter plot will be highlighted and the corresponding
cells in the image will be marked by colored boxes.
To de-select the cells in the image, right-click in the plot area and select
‘Delete Image Overlay’ or right-click on the image overlay button in the
image window and select ‘Delete Image Overlay’.
Gating Cell Populations for Inclu-
sion/Exclusion in Analysis
Particular sub-populations of observed events can be either included or
excluded in the data analysis using gating options. The sub-populations are
those defined by the polygons, quadrants and markers.