ANKOM XT10 Series - Manual (Page 10)

Operator’s Manual
pg. 10
Rev F 12/08/14
Fat Extraction Calculations
Crude Fat Calculation
Crude Fat contained within a food or feed sample can be calculated using the following formula:
% Crude Fat = 100 x (W
2
– W
3
)
W
1
Where: W
1
= Original weight of sample
W
2
= Weight of pre-extraction dried sample and filter bag
W
3
= Weight of dried sample and filter bag after extraction
For Liquid Samples, W
2
and W
3
in the equation above will include the weight of Diatomaceous Earth (DE).
For meat samples with > 15% fat and plant samples with > 20% fat:
% Crude Fat = 100 x ((W
2
– W
4
) – W
3
)
W
1
Where: W
4
= Weight of the weigh tin
Total Fat Calculation
If hydrolysis is done prior to fat extraction, then Total Fat contained within a food or feed sample can be calculated
using the following formula:
% Total Fat = 100 x (W
2
– (W
3
+ (C
1
– C
2
)))
W
1
Where: W
1
= Original weight of sample
W
2
= Weight of dried sample, filter bag, and Diatomaceous Earth (DE) after hydrolysis
W
3
= Weight of dry extracted sample, filter bag, and DE
C
1
= Blank filter bag dry weight after hydrolysis
C
2
= Blank filter bag weight after extraction
Fat Extraction Support Items
The following support items are needed to perform the fat extraction procedure:
Item Recommended Product
Electronic Balance with four-place readout ANKOM #TB Balance Hardware
ANKOM #TBS Balance Software
Filter Bags ANKOM #XT4
Bag Holder (used for adding sample to an empty filter bag) ANKOM #101.2
Heat Sealer for sealing the filter bags ANKOM #1915 (120V), #1920 (220V)
Solvent Resistant Marker ANKOM #F08
Desiccant Pouch or Weigh Tin Desiccator for drying ANKOM #X45, #X49
Dryer (capable of temperatures from 60-120°C) - optional ANKOM #RD (120V), #RDI (220V)
Oven for drying (capable of maintaining 102°C±2°)
Ventilated oven trays to allow for sufficient oven air flow
Weigh Tins (used with samples of > 20% fat)
Sample
Spoon